Semaglutide Reconstitution Calculator & Dosage Chart
Also known as: Ozempic, Wegovy, GLP-1
In short: mix a 5 mg vial of Semaglutide with 2 ml of bacteriostatic water. That gives 2.50 mg/ml, so a typical 500 mcg dose is 20 units on a U-100 insulin syringe — about 10 doses per vial. Reported protocols range 250 mcg–2.4 mg, 1x weekly, titrated up over months.
Semaglutide is a GLP-1 receptor agonist — the active compound in Ozempic and Wegovy — used for blood-sugar control and weight management. Compounded and research versions come as lyophilized powder that must be reconstituted, which is where dosing math errors are most common. Full overview: what is Semaglutide? →
⚠ Titration matters: published schedules start at 0.25 mg weekly and increase every 4 weeks. Doses here are reference points, not a schedule.
Calculate your exact dose
Typical range: 250 mcg–2.4 mg, 1x weekly, titrated up over months
Semaglutide mixing chart — 5 mg vial
Units to draw for a 500 mcg dose at different water amounts. More water never changes the dose — only the volume you measure.
| Water added | Concentration | 500 mcg dose = | Per unit |
|---|---|---|---|
| 1 ml | 5.00 mg/ml | 10 units | 50.0 mcg |
| 2 ml ← typical | 2.50 mg/ml | 20 units | 25.0 mcg |
| 2.5 ml | 2.00 mg/ml | 25 units | 20.0 mcg |
| 3 ml | 1.67 mg/ml | 30 units | 16.7 mcg |
| 5 ml | 1.00 mg/ml | 50 units | 10.0 mcg |
Semaglutide reconstitution by vial size — 2 mg / 5 mg / 10 mg
How much water for each vial size, and what a 500 mcg dose becomes in syringe units. Bigger vials hold more doses; they do not change the dose.
| Vial | Water | Concentration | 500 mcg dose = | Doses per vial |
|---|---|---|---|---|
| 2 mg | 1 ml | 2.00 mg/ml | 25 units | 4 |
| 2 mg | 2 ml | 1.00 mg/ml | 50 units | 4 |
| 2 mg | 3 ml | 0.67 mg/ml | 75 units | 4 |
| 5 mg | 1 ml | 5.00 mg/ml | 10 units | 10 |
| 5 mg | 2 ml | 2.50 mg/ml | 20 units | 10 |
| 5 mg | 3 ml | 1.67 mg/ml | 30 units | 10 |
| 10 mg | 1 ml | 10.00 mg/ml | 5.0 units | 20 |
| 10 mg | 2 ml | 5.00 mg/ml | 10 units | 20 |
| 10 mg | 3 ml | 3.33 mg/ml | 15 units | 20 |
Protocol reference
FAQ
How much bacteriostatic water do you add to Semaglutide?+
Most protocols mix a 5 mg vial of Semaglutide with 2 ml of bacteriostatic water. This gives 2.50 mg/ml, so a typical 500 mcg dose is 20 units on a U-100 insulin syringe. More or less water is fine — it only changes the volume you draw, not the dose.
What is a typical Semaglutide dose?+
Commonly reported Semaglutide protocols use 250 mcg to 2.4 mg, 1x weekly, titrated up over months, administered via subcutaneous injection. These are reference figures from published protocols, not medical advice — individual use should be guided by a healthcare provider.
How many doses are in a 5 mg vial of Semaglutide?+
At 500 mcg per dose, a 5 mg vial contains about 10 doses, regardless of how much water you add.
How should Semaglutide be stored?+
Reconstituted: refrigerate; typically used within 4–8 weeks. Never freeze.
Related peptides
Educational reference only. Values reflect commonly published research protocols and are not medical advice or a recommendation to use any compound. Semaglutide may not be approved for human use in your jurisdiction.